Lili Jiang
Chinese Journal of Aesthetic Medicine · 2010
Abstract
Objective To investigate the effects of resveratrol on melanin and Langerhans cells from solar simulator ultraviolet irradiation (ssUVR).Materials and Methods Eleven healthy female volunteers were enrolled in this study.Six sites on the non-exposed back skin were marked for the experiment.Sites 1～4 were applied with resveratrol (Res)+antioxidant (Aox),antioxidant,resveratrol,vehicle,and followed by ssUVR at a dosage of 1.5 minimal erythema dose (MED) 30 min later.Site 5 received ssUVR without any test material (positive control)and site 6 received neither ssUVR nor test materials (negative control).The skin biopsies were taken 24 hours after the last ssUVR.The specimens were stained with Fontana Manna staining and immunohistochemistry staining of Monoclonal Antibody-CD1a.Results The measured percentage (%) and average optical density of MCI increased significantly on UV only site and Vehicle+UV site,much higher than on Res+Aox+UV site,Aox+UV site and Res+UV site (P0.05).The density of Langerhans cells decreased dramatically on UV only and Vehicle+UV site,significant more than on Res+Aox+UV site,Aox+UV site and Res+UV site (P0.05).Conclusions Resveratrol,antioxidant or their combination were effective in protecting the skin from ssUVR-induced melanin increase and Langerhans cells depletion.