Effect of Grape Seed Procyanidin Extract on number and morphous of Langerhans cells

Wei Liu

Chinese Journal of Aesthetic Medicine · 2009

Abstract

Objective In this study,the effect of Grape Seed Procyanidin Extract(GSPE) on Langerhans cells from ultraviolet irradiation was investigated in skin by solar-simulator radiation.Methods Areas of skin of normal volunteers were divided into four sites: normal untreated skin(G1 Group),skin treated with 2-MED UV radiation(G2 Group),or skin treated with vehicle(G3 Group) or GPSE(G4 Group) 30minutes before 2-MED solar simulated radiation.Skin areas of different sites were examined immunohistochemically for CD1a+ Langerhans cells.Results On immunohistochemical examination,CD1a+ Langhans cells was obviously reduced in UV irradiated skin,while topically applied GSPE before UV radiation reconstituted the Langerhans cells compared with skin treated with UV radiation only.We also found more dendrites and longer dendrites in most of Langerhans cells in G4 Group,which may be signs of immune activation.Conclusions GSPE may protect skin against photo immunosuppression partly through reconstituting Langerhans cells.